Mani Panchatcharam, Ph.D.
Ph.D., 2006, University of Madras
Department of Cellular Biology and Anatomy
F8-22 Biomedical Research Institute
1501 Kings Highway
LSU Health Sciences Center
Shreveport, LA 71130
Major Research Interests: Dr. Panchatcharam’s research focuses on lipids which play a major factor in blocking blood vessels leading to heart attack. Among those major lipids, Lysophosphatidic acid (LPA) is been regulated and controlled by Lipid Phosphate Phosphatases (LPPs) which plays a pivotal role. Our laboratory focuses on the role of LPA-LPP axis involved in cardiac function.
Ongoing cardiovascular-related projects: 1. Oxidative stress and Lysolipid signaling in cardiovascular disease Brief description: A common single nucleotide polymorphism (SNP) in the gene Ppap2b associates with risk of coronary artery disease (CAD). PPap2b encodes the enzyme lipid phosphate phosphatase (LPP) 3 that degrades and thereby may attenuate lysophosphatidic acid (LPA) responsiveness of cells. The bioactive lipid mediators LPA regulate a broad range of physiological processes. Genetic and pharmacological evidence implicates them as mediators of vascular inflammation, injury responses, blood pressure, and immune function. However, a pathophysiologic role for LPP3 in vascular cells remains to be established. Names of collaborators / collaborating institutions: Dr. William Mayhan – LSUHSC Shreveport; Dr. Daniel Neil Granger – LSUHSC Shreveport; Dr. Christopher Kevil – LSUHSC Shreveport; Dr. Jonathan Steven Alexander – LSUHSC Shreveport; Dr. Kevin McCarthy – LSUHSC Shreveport; Dr. Sumitra Miriyala – LSUHSC Shreveport; Dr. Anthony Wayne Orr – LSUHSC Shreveport; Dr. Ikuo Tsunoda – LSUHSC Shreveport; Dr. Sushil Jain – LSUHSC Shreveport; Dr. Hong Sun – LSUHSC Shreveport; Dr. Susan Smyth – University of Kentucky, Lexington; Dr. Diana Escalante-Alcalde – Departamento de Neurociencias, Universidad Nacional Autonoma de Mexico, Mexico; Dr. Ines Batinic- Haberle – Duke University, Durham; Dr. Daret St Clair – University of Kentucky, Lexington; Dr. Mary Vore – University of Kentucky, Lexington; Dr. Eric Kelley – University of Pittsburgh, Pittsburgh. Funding source: Scientist development grant (10SDG4190036) from the American Heart Association; LSUHSC-S Research Grant-In- Aid award
Planned cardiovascular-related projects:
- Regulation of Vascular Permeability and Angiogenesis by Lipid Phosphate Phosphatase
- Functional validation of PPAP2B as a novel risk factor for coronary artery disease.
- Regulation of lysolipid signaling by lipid phosphate phosphatase 3 in vascular responses to injury.
- Functional validation of lipid phosphate phosphatase 3 in myocardium.
- Regulation of LPP3 in blood brain barrier.
Methods and Available Resources:
- Isolation of primary cell lines from mouse and rats
- Preparation of plasmids for Lentiviral production
- Transfection of cells (Viral and shRNA plasmids)
- Western blot (LICOR(Fluorescence) and ECL method)
- Site directed mutagenesis
- Surface Plasmon resonance (Protein- DNA binding)
- Mitochondrial Bioenergetics – (Seahorse analyzer; Clark’s Electrode)
- Mitochondrial Activity assays
- Mass spectrophometry.
- Mice models involving carotid artery ligation, femoral artery ligation, coronary artery ligation, osmotic pump and matrigel gel implantation.