Sumitra Miriyala, Ph.D.

Sumitra Miriyala, Ph.D.
Assistant Professor – Research
Ph.D., 2005, University of Madras
Department of Cellular Biology and Anatomy
F8-13 Biomedical Research Institute
1501 Kings Highway
LSU Health Sciences Center
Shreveport, LA 71130
Phone: 318.675.6940
Fax: 318.675.5889
E-mail:smiriy@lsuhsc.edu

Major Research Interests: Dr. Miriyala’s research involves evaluating genetic abnormalities of antioxidant enzymes, the mechanisms regulating gene expression and the impact these alterations have on the ability of humans to cope with oxidative stress. Her focuses are on the novel mechanism by which oxidative stress activates mitochondrial retrograde signaling in cardiac tissues under life and death conditions.

Ongoing cardiovascular-related projects: 1. Role of Manganese superoxide dismutase in cardiovascular diseases Brief description: Dr. Miriyala’s research involves evaluating genetic abnormalities of antioxidant enzymes, the mechanisms regulating gene expression and the impact these alterations have on the ability of humans to cope with oxidative stress. Her focuses are on the novel mechanism by which oxidative stress activates mitochondrial retrograde signaling in cardiac tissues under life and death conditions Names of collaborators / collaborating institutions: Dr. Christopher Kevil – LSUHSC Shreveport; Dr. Jonathan Steven Alexander – LSUHSC Shreveport; Dr. Sumitra Miriyala – LSUHSC Shreveport; Dr. Anthony Wayne Orr – LSUHSC Shreveport; Dr. Ikuo Tsunoda – LSUHSC Shreveport; Dr. Diana Escalante-Alcalde – Departamento de Neurociencias, Universidad Nacional Autonoma de Mexico, Mexico; Dr. Ines Batinic- Haberle – Duke University, Durham; Dr. Daret St Clair – University of Kentucky, Lexington; Dr. Mary Vore – University of Kentucky, Lexington. Funding source: LSHUSC: Grant-In-Aid (PI); Scientist development grant (10SDG4190036) from the American Heart Association (Collaborator)

Planned cardiovascular-projects:

      1. Functional validation of MnSOD in Coronary Artery Disease
      2. Identification of the role of MnSOD in diabetic restenosis
      3. Oxidative stress mediated retrograde signaling: The Emerging Role of Mitochondria in cardiovascular disease

Methods and Available Resources:

      • Isolation of primary cell lines from mouse and rats
      • Immunocytochemistry/Immunohistochemistry
      • Preparation of plasmids for Lentiviral production
      • Transfection of cells (Viral and shRNA plasmids)
      • Western blot (LICOR(Fluorescence) and ECL method)
      • Co-Immunoprecipitation
      • Site directed mutagenesis
      • Surface Plasmon resonance (Protein- DNA binding)
      • Mitochondrial Bioenergetics – (Seahorse analyzer; Clark’s Electrode)
      • Mitochondrial Activity assays

Publications: http://www.ncbi.nlm.nih.gov/pubmed/?term=sumitra+miriyala